This meta-analytic review (k = 5-10; N = 258-895) examined links between attachment insecurity and physiological activity at baseline and in response to interpersonal stress elicited by separation-reunion procedures in the early life course (1-5 years). Insecurity was trivially, nonsignificantly associated with baseline physiological activity (heart rate [HR]: g = -.06; respiratory sinus arrhythmia [RSA]: g = -.06; cortisol: g = .01) and nonsignificantly associated with physiological reactivity to separation from parents (HR: g = -.001; RSA: g = .24). However, insecurity was moderately associated with heightened RSA (g = .26) and cortisol (g = .27) reactivity upon reunion with parents. Findings provide insight into the biobehavioral organization of attachment, suggesting that early insecurity is associated with heightened physiological reactivity to interpersonal stress. [ABSTRACT FROM AUTHOR]