The effect of recording interval lenght on behavioral assessment using the forced swimming test

• Paloma Álvarez-Suárez ^[1] ; María Banqueri ^[1] ; Marina Vilella ^[1] ; Marta Méndez López ^[1] ; Jorge Luis Arias Pérez ^[1]
  1. [1] Universidad de Oviedo

     Universidad de Oviedo

     Oviedo, España

     
• Localización: Revista iberoamericana de psicología y salud, ISSN 2171-2069, Vol. 6, Nº. 2, 2015, págs. 90-95
• Idioma: inglés
• DOI: 10.1016/j.rips.2015.04.004
• Enlaces
  • Texto completo
• Resumen
  • español

    El test de natación forzada es un método utilizado para evaluar el comportamiento depresivo en roedores. Las modificaciones del procedimiento del test de natación forzada desarrollado por Porsolt y su influencia en sus resultados es un tema que suscita controversia y ha sido analizado en numerosos estudios. El comportamiento del animal se analiza generalmente mediante el registro de intervalos parciales, dividiendo el tiempo total de grabación en intervalos iguales y registrando manualmente la conducta predominante durante ese tiempo. A pesar de la influencia del método de registro en los resultados posteriores, esta cuestión no se ha analizado ni normalizado. El objetivo de este estudio fue comprobar si la representatividad de los datos se ve afectada por la longitud del intervalo de observación, registrando a 3, 5 y 10 seg la conducta (inmovilidad, natación y escalada) de los mismos sujetos. Se utilizo una muestra no patológica de ratas Wistar macho y hembra adultas. Los resultados mostraron que no existen diferencias significativas entre estos intervalos analizados en la prueba de natación forzada para las 3 principales conductas registradas.

  • English

    The forced swimming test is a method used in the assessment of depressive-like behavior in rodents. Changes in the original forced swimming test procedure developed by Porsolt et al. and their influence in the results is a controversial issue and has been discussed in many studies. Animal's behavior is usually recorded by partial interval recording, dividing the total recording time into equal intervals and manually recording the predominant behavior. Despite the influence of the recording method in the subsequent results, this issue has not been further studied nor normalized. The aim of this study was to assess whether the representativeness of the data is influenced by the recording interval length, by recording behaviors (immobility, swim and climbing) in the same subjects at 3, 5 and 10 s recording intervals. We used a non-pathological sample of male and female adult Wistar rats. Our results show no differences in the use of these three recording intervals in the registration method of the forced swimming test, for the main three behaviors measured.

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