Risperidone regulates Dopamine D2-like receptors expression in rat brain in a time-dependent manner

• Autores: Ni Peiyan, Liang Linhui, Wang Yingcheng, Wei Jinxue, Gu Xiaochu, Zhao liansheng, Ma Xiaohong, Li Tao
• Localización: European journal of psychiatry, ISSN 0213-6163, Vol. 29, Nº 1, 2015, págs. 33-43
• Idioma: inglés
• DOI: 10.4321/s0213-61632015000100003
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• Resumen

  • Background and Objectives:

    Antipsychotics can elicit dopamine super- sensitivity by up-regulation of D2-like receptors (DRD2, DRD3, and DRD4) expression.

    Nevertheless, the expression profile of dopamine D2-like receptors in different brain re- gions and peripheral blood mononuclear cells (PBMCs), and changes following risperi- done administration were still unclear. In this study, we would investigate the expression of D2-like receptors mRNA in different brain regions and the peripheral blood mononu- clear cells (PBMCs) in rats after 2, 6 weeks risperidone administration.

    Methods:

    The experimental rats were given risperidone (0.25mg/kg/day, i.p.), and the control rats were given 0.9% NaCl. The rats were sacrificed at 0 week, 2 weeks and 6 weeks after the drug administration. Expression of the dopamine D2-like receptors was quanti- fied by Real-time PCR method.

    Results : Dopamine D2-like receptors expressed in all the examined regions of rat brain.

    Their expression significantly increased 2weeks after risperidone administration in differ- ent brain regions. However, the changed expression of DRD2 and DRD3 turned back to the basal level 6weeks later, while the increased DRD4 expression remained in left pari- etal cortex. Meanwhile, DRD2 and DRD3 but not DRD4 expressed in PBMCs, however, the risperidone could not affect their expression.

    Conclusions:

    The risperidone could change the dopamine D2-like receptors expression in a time-dependent manner in different brain regions, which might guide the clinical use in the near future