Reduction of inflammatory pain in female rats after NE2B NMDA cortical antagonism

• Autores: Carol Vasques, Melany Sánchez, Jairo Herrera, Gabriel Quintero
• Localización: Psicothema, ISSN-e 1886-144X, ISSN 0214-9915, Vol. 24, Nº. 2, 2012, págs. 296-301
• Idioma: inglés
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• Resumen

  • Reducción del dolor infl amatorio en ratas hembras tras el antagonismo cortical de NR2B NMDA.

    Estudios han demostrado que los receptores de N-methyl-D-aspartato (NMDA) participan en el procesamiento del dolor en diferentes niveles del SNC. Este estudio empleó ratas hembras adultas Wistar para evaluar el antagonismo de la subunidad NR2B de NMDA en los procesos de dolor fásico y tónico.

    Se implantaron cánulas corticalmente con la cirugía estereotáxica y tras una semana de recuperación se realizaron pruebas conductuales. Se evaluaron los efectos del fármaco en la coordinación motora en el aparato de barra giratoria. Además, las ratas realizaron la prueba de latencia de retirada de la pata a un estímulo termal nocivo. Posteriormente, las ratas realizaron la prueba de formalina. Las ratas hembras que recibieron el antagonista de NR2B, Ro 25-6981, antes y después de la inyección de formalina denotaron respuestas de dolor signifi cativamente menores en comparación con los controles (p<0.05).

    En contraste, no se encontraron diferencias signifi cativas en la prueba de dolor fásico (Hargreaves) y la prueba de barra giratoria. En conjunto, estos resultados sugieren que el antagonismo cortical de la subunidad NR2B de los receptores NMDA es capaz de reducir los niveles de dolor infl amatorio, antes y después de la inyección de formalina en las distintas fases del ciclo estral.

• Referencias bibliográficas
  • Abbott, F.V., & Melzack, R. (1982). Brainstem lesions dissociate neural mechanisms of morphine analgesia in different kinds of pain. Brain...
  • Abbott, F.V., Melzack, R., & Samuel, C. (1982). Morphine analgesia in tail-flick and formalin pain tests is mediated by different neural...
  • Alreja, M., Mutalik, P., Nayar, U., & Manchanda, S.K. (1984). The formalin test: A tonic pain model in the primate. Pain, 20(1), 97-105.
  • Becker, J.B., Arnold, A.P., Berkley, K.J., Blaustein, J.D., Eckel, L.A., Hampson, E., et al. (2005). Strategies and methods for research on...
  • Boyce, S., Wyatt, A., Webb, J.K., O'Donnell, R., Mason, G., Rigby, M., et al. (1999). Selective NMDA NR2B antagonists induce antinociception...
  • Bryant, C.D., Eitan, S., Sinchak, K., Fanselow, M.S., & Evans, C.J. (2006). NMDA receptor antagonism disrupts the development of morphine...
  • Carter, R.J., Morton, J., & Dunnett, S.B. (2001). Motor coordination and balance in rodents. Current Protocols in Neuroscience, Chapter...
  • Coderre, T.J., & Melzack, R. (1992). The contribution of excitatory amino acids to central sensitization and persistent nociception after...
  • Chang, C., & Shyu, B.C. (2001). A fMRI study of brain activations during non-noxious and noxious electrical stimulation of the sciatic...
  • Dubuisson, D., & Melzack, R. (1977). Analgesic brain stimulation in the cat: Effect of intraventricular serotonin, norepinephrine, and...
  • Eaton, S.A., & Salt, T.E. (1990). Thalamic NMDA receptors and nociceptive sensory synaptic transmission. Neuroscience Letters, 110(3),...
  • Fischer, G., Mutel, V., Trube, G., Malherbe, P., Kew, J.N., Mohacsi, E., et al. (1997). Ro 25-6981, a highly potent and selective blocker...
  • Hargreaves, K., Dubner, R., Brown, F., Flores, C., & Joris, J. (1988). A new and sensitive method for measuring thermal nociception in...
  • Hunskaar, S., Fasmer, O.B., & Hole, K. (1985). Formalin test in mice, a useful technique for evaluating mild analgesics. Journal of Neuroscince...
  • Hunter, J.C., & Singh, L. (1994). Role of excitatory amino acid receptors in the mediation of the nociceptive response to formalin in...
  • Johansen, J.P., & Fields, H.L. (2004). Glutamatergic activation of anterior cingulate cortex produces an aversive teaching signal. Nature...
  • Juni, A., Klein, G., Kowalczyk, B., Ragnauth, A., & Kest, B. (2008). Sex differences in hyperalgesia during morphine infusion: Effect...
  • Kavaliers, M., & Choleris, E. (1997). Sex differences in N-methyl-Daspartate involvement in kappa opioid and non-opioid predator-induced...
  • Kim, S.S., Descalzi, G., & Zhuo, M. (2010). Investigation of molecular mechanism of chronic pain in the anterior cingulate cortex using...
  • Lariviere, W.R., Sattar, M.A., & Melzack, R. (2006). Inflammation-susceptible Lewis rats show less sensitivity than resistant Fischer...
  • Lee, I.O., & Jeong, Y.S. (2002). Effects of different concentrations of formalin on paw edema and pain behaviors in rats. Journal of Korean...
  • Li, X.Y., Ko, H.G., Chen, T., Descalzi, G., Koga, K., Wang, H., et al. (2010). Alleviating neuropathic pain hypersensitivity by inhibiting...
  • Marcondes, F.K., Bianchi, F.J., & Tanno, A.P. (2002). Determination of the estrous cycle phases of rats: Some helpful considerations....
  • McRoberts, J.A., Li, J., Ennes, H.S., & Mayer, E.A. (2007). Sex-dependent differences in the activity and modulation of N-methyl-d-aspartic...
  • Mogil, J.S., Davis, K.D., & Derbyshire, S.W. (2010). The necessity of animal models in pain research. Pain, 151(1), 12-17.
  • Mogil, J.S., Sternberg, W.F., Kest, B., Marek, P., & Liebeskind, J.C. (1993). Sex differences in the antagonism of swim stress-induced...
  • Quintero, G.C., Erzurumlu, R.S., & Vaccarino, A.L. (2007). Decreased pain response in mice following cortex-specific knockout of the N-methyl-D-aspartate...
  • Ryan, S.M., Watkins, L.R., Mayer, D.J., & Maier, S.F. (1985). Spinal pain suppression mechanisms may differ for phasic and tonic pain....
  • Smith, P.F. (2003). Therapeutic N-methyl-D-aspartate receptor antagonists: Will reality meet expectation? Current Opinion in Investigational...
  • South, S.M., Kohno, T., Kaspar, B.K., Hegarty, D., Vissel, B., Drake, C.T., et al. (2003). A conditional deletion of the NR1 subunit of the...
  • Tuor, U.I., Malisza, K., Foniok, T., Papadimitropoulos, R., Jarmasz, M., Somorjai, R., et al. (2000). Functional magnetic resonance imaging...
  • Vaccarino, A.L., Clemmons, H.R., Mader, G.J., Jr., & Magnusson, J.E. (1997). A role of periaqueductal grey NMDA receptors in mediating...
  • Vaccarino, A.L., Marek, P., Kest, B., Weber, E., Keana, J.F., & Liebeskind, J.C. (1993). NMDA receptor antagonists, MK-801 and ACEA-1011,...
  • Vaccarino, A.L., & Melzack, R. (1989). Analgesia produced by injection of lidocaine into the anterior cingulum bundle of the rat. Pain,...
  • Vaccarino, A.L., & Melzack, R. (1992). Temporal processes of formalin pain: Differential role of the cingulum bundle, fornix pathway and...
  • van Dellen, A., Cordery, P.M., Spires, T.L., Blakemore, C., & Hannan, A.J. (2008). Wheel running from a juvenile age delays onset of specific...
  • Wei, F., Li, P., & Zhuo, M. (1999). Loss of synaptic depression in mammalian anterior cingulate cortex after amputation. The Journal of...
  • Wei, F., Qiu, C.S., Kim, S.J., Muglia, L., Maas, J.W., Pineda, V.V., et al. (2002). Genetic elimination of behavioral sensitization in mice...
  • Wei, F., Wang, G.D., Kerchner, G.A., Kim, S.J., Xu, H.M., Chen, Z.F., et al. (2001). Genetic enhancement of inflammatory pain by forebrain...
  • Wu, L.J., & Zhuo, M. (2009). Targeting the NMDA receptor subunit NR2B for the treatment of neuropathic pain. Neurotherapeutics, 6(4),...
  • Young, E.A., & Becker, J.B. (2009). Perspective: Sex matters: Gonadal steroids and the brain. Neuropsychopharmacology, 34(3), 537-538.
  • Zhuo, M. (2006). Molecular mechanisms of pain in the anterior cingulated cortex. Journal of Neuroscience Research, 84(5), 927-933.
  • Zhuo, M. (2007). A synaptic model for pain: Long-term potentiation in the anterior cingulate cortex. Molecules and Cells, 23(3), 259-271.
  • Zimmermann, M. (1983). Ethical guidelines for investigations of experimental pain in conscious animals. Pain, 16(2), 109-110.