Brain c-Fos immunocytochemistry and cytochrome oxidase histochemistry after a fear conditioning task

• Nélida María Conejo Jiménez ^[1] ; Héctor González Pardo ^[1] ; Matías López Ramírez ^[1] ; Raúl Cantora ^[1] ; Jorge Luis Arias Pérez ^[1]
  1. [1] Universidad de Oviedo

     Universidad de Oviedo

     Oviedo, España

     
• Localización: Psicothema, ISSN-e 1886-144X, ISSN 0214-9915, Vol. 19, Nº. 2, 2007, págs. 295-301
• Idioma: inglés
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• Dialnet Métricas: 1 Cita
• Resumen

  • Inmunocitoquímica c-Fos e histoquímica citocromo oxidasa cerebral tras una tarea de condicionamiento al miedo. Se analiza la implicación de la amígdala basolateral y medial en el condicionamiento al miedo empleando diferentes marcadores de activación neuronal. El método que describimos es una combinación de la histoquímica citocromo oxidasa (CO) y la inmunocitoquímica c-Fos en secciones de tejido cerebral. La conducta de inmovilización se usó como índice del condicionamiento auditivo y contextual. Las puntuaciones obtenidas fueron significativamente mayores en ratas expuestas a emparejamientos tono-descarga en un ambiente distintivo (grupo COND), comparadas a las obtenidas en ratas que nunca recibieron descargas eléctricas (UNCD). La actividad CO se incrementó en la amígdala medial y basolateral del grupo COND. Sin embargo, la expresión c-Fos en estos núcleos fue inferior en el grupo COND comparado al grupo UNCD. Además, los niveles de la proteína c-Fos fueron más altos en la amígdala medial del grupo UNCD. Nuestros resultados muestran que los núcleos amigdalinos podrían jugar diferentes papeles en el condicionamiento al miedo auditivo y contextual.

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